imc measurement (fluidigm)
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imc measurement
Imc Measurement, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imc+measurement/Hyperion+Imaging+Mass+Cytometry+System/pmc12317575-127-27-34
Average 93 stars, based on 1523 article reviews
Imc Measurement, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imc+measurement/Hyperion+Imaging+Mass+Cytometry+System/pmc12317575-127-27-34
Average 93 stars, based on 1523 article reviews
imc measurement - by Bioz Stars,
2026-09
93/100 stars
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Staining:Article Title: Sensitive detection of minimal residual disease and immunotherapy targets by multi-modal bone marrow analysis in high-risk neuroblastoma – a multi-center study Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Meanwhile, antibody cocktails were retrieved from −80 °C and thawed for 15 min at 4 °C before staining overnight at 4 °C. .. The following day, slides were washed twice (5 min per wash) in TBS, stained with the DNA intercalator Iridium (Fluidigm) and then dried with compressed air before Article Title: Cross-Species Analysis Identifies Conserved Transcriptional Mechanisms of Neutrophil Maturation Article Snippet: Samples were thawed 562 for 15 min at RT, washed twice in TBS, fixed in 4% PFA (Carl Roth) at 4°C for 30 min, washed again twice 563 in TBS and then blocked with 3% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. 564 Samples were incubated overnight at 4°C with diluted at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the 565 following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with 566 pressured air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Article Title: Cross-species analysis identifies conserved transcriptional mechanisms of neutrophil maturation Article Snippet: Samples were thawed for 15 min at RT, washed twice in TBS, fixed in 4% PFA (Carl Roth) at 4°C for 30 min, washed again twice in TBS and then blocked with 3% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit) diluted at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation. Article Snippet: Samples were thawed for 15min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Imaging:Article Title: Sensitive detection of minimal residual disease and immunotherapy targets by multi-modal bone marrow analysis in high-risk neuroblastoma – a multi-center study Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Meanwhile, antibody cocktails were retrieved from −80 °C and thawed for 15 min at 4 °C before staining overnight at 4 °C. .. The following day, slides were washed twice (5 min per wash) in TBS, stained with the DNA intercalator Iridium (Fluidigm) and then dried with compressed air before Article Title: Cross-Species Analysis Identifies Conserved Transcriptional Mechanisms of Neutrophil Maturation Article Snippet: Samples were thawed 562 for 15 min at RT, washed twice in TBS, fixed in 4% PFA (Carl Roth) at 4°C for 30 min, washed again twice 563 in TBS and then blocked with 3% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. 564 Samples were incubated overnight at 4°C with diluted at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the 565 following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with 566 pressured air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Article Title: Cross-species analysis identifies conserved transcriptional mechanisms of neutrophil maturation Article Snippet: Samples were thawed for 15 min at RT, washed twice in TBS, fixed in 4% PFA (Carl Roth) at 4°C for 30 min, washed again twice in TBS and then blocked with 3% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit) diluted at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation. Article Snippet: Samples were thawed for 15min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Software:Article Title: Sensitive detection of minimal residual disease and immunotherapy targets by multi-modal bone marrow analysis in high-risk neuroblastoma – a multi-center study Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Meanwhile, antibody cocktails were retrieved from −80 °C and thawed for 15 min at 4 °C before staining overnight at 4 °C. .. The following day, slides were washed twice (5 min per wash) in TBS, stained with the DNA intercalator Iridium (Fluidigm) and then dried with compressed air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation Article Snippet: Samples were thawed for 15 min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30 min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween-20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before Article Title: Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation. Article Snippet: Samples were thawed for 15min at RT, fixed in 4% PFA (Carl Roth) at 4 °C for 30min, washed twice in TBS and then blocked with 2% BSA (Carl Roth) and 0.1% Tween-20 (Merck) in TBS at RT for 1 h. Samples were incubated overnight at 4 °C with diluted CD15 antibody (BioLegend; catalog number: 301902; clone: HI98) at 5 μg/ml in 0.1% Tween20 in TBS. .. On the following day, samples were stained with the DNA intercalator Iridium (Fluidigm) and then dried with pressured air before |